Fig. 2. EC-specific HDAC2 overexpression attenuates elevated vascular stiffness in atherogenic mice. (A) Aortas from wild-type (WT, C57BL/6 control) and HDAC2-Tg (Tie2 promoter) mice were immunoblotted (Ib) with HDAC2 and GAPDH antibodies. *p<0.05, n=3. (B, C) C57BL/6 and HDAC2-Tg mice were induced to develop atherosclerosis via liver-specific overexpression of PCSK9 and a high-fat diet. (B) Pulse wave velocity was higher in the C57BL/6 WT mice than in the HDAC2-Tg mice. *p<0.05 vs C57BL6 baseline, #p<0.05 vs C57BL/6 PCSK9AAV, n=15. (C) Mean arterial pressure did not differ between the C57BL/6 and HDAC2-Tg mice at baseline or after the atherogenic regimen. ns, not significant.